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KMID : 0624620170500090460
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BMB Reports
2017 Volume.50 No. 9 p.460 ~ p.465
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Effects of PEP-1-FK506BP on cyst formation in polycystic kidney disease
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Jo Hyo-Sang
Eum Won-Sik
Park Eun-Young
Ko Je-Young
Kim Do-Yeon
Kim Dae-Won
Shin Min-Jea
Son O-Ra
Cho Su-Bin
Park Jung-Hwan
Lee Chi-Hern
Yeo Eun-Ji
Yeo Hyeon-Ji
Choi Yeon-Joo
Youn Jong-Kyu
Cho Sung-Woo
Park Jin-Seu
Park Jong-Hoon
Choi Soo-Young
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Abstract
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Polycystic kidney disease (PKD) is one of the most common inherited disorders, involving progressive cyst formation in the kidney that leads to renal failure. FK506 binding protein 12 (FK506BP) is an immunophilin protein that performs multiple functions, including regulation of cell signaling pathways and survival. In this study, we determined the roles of PEP-1-FK506BP on cell proliferation and cyst formation in PKD cells. Purified PEP-1-FK506BP transduced into PKD cells markedly inhibited cell proliferation. Also, PEP-1-FK506BP drastically inhibited the expression levels of p-Akt, p-p70S6K, p-mTOR, and p-ERK in PKD cells. In a 3D-culture system, PEP-1-FK506BP significantly reduced cyst formation. Furthermore, the combined effects of rapamycin and PEP-1-FK506BP on cyst formation were markedly higher than the effects of individual treatments. These results suggest that PEP-1-FK506BP delayed cyst formation and could be a new therapeutic strategy for renal cyst formation in PKD.
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KEYWORD
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Cyst formation, PEP-1-FK506BP, Polycystic kidney diseases, Protein therapy, 3D culture
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